LOCUS       sequence-54438-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Gallegos LL, Kunkel MT, Newton AC
  TITLE     Targeting protein kinase C activity reporter to discrete 
            intracellular regions reveals spatiotemporal differences in 
            agonist-dependent signaling.
  JOURNAL   J Biol Chem. 2006 Oct 13. 281(41):30947-56.
  PUBMED    16901905
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 Oct 13. 281(41):30947-56."
COMMENT     Sequence Label: GolgiCFP
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(167..186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(168..186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(231..434)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(260..280)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        435..738
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agctctcgcc cttgctcaca ccacctccac ctcctccacc acctccacct gccggggtgg
       61 ctgggccctg cttgccgcac aatccaagtc ccagacccag tccaagtccg cagggtggcc
      121 caggctcctg ggccacgctc ttcaagttgc ccataagctt gggtctccct atagtgagtc
      181 gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt
      241 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      301 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      361 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      421 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      481 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      541 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      601 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
      661 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      721 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
      781 gattactatt a
//
