LOCUS       sequence-54558-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Mansour SJ, Matten WT, Hermann AS, Candia JM, Rong S, Fukasawa K, 
            Vande Woude GF, Ahn NG
  TITLE     Transformation of mammalian cells by constitutively active MAP 
            kinase kinase.
  JOURNAL   Science. 1994 Aug 12;265(5174):966-70.
  PUBMED    8052857
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1994-08-12"; volume: "265(5174)"; pages: "966-70"
COMMENT     Sequence Label: pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..68
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 cgtcagatct ctagaagctg ggtaccacgc gtcatatggc ctacccatac gatgttccag
       61 attacgctag cctgggatcc ggcggagttg gaagcgcgtt acccgggtcc aaaatgccca
      121 agaagaagcc gacgcccatc cagctgaacc cggcccccga cggctctgca gttaacggga
      181 ccagctctgc ggagaccaac ttggaggcct ttcttaccca gaagcagaag gtgggagaac
      241 tgaaggatga cgactttgag aagatcagtg agctgggggc tggcaatggc ggtgtggtgt
      301 tcaaggtctc ccacaagcct tctggcctgg tcatggccag aaagctaatt catctggaga
      361 tcaaacccgc aatccggaac cagatcataa gggagctgca ggttctgcat gagtgcaact
      421 ctccgtacat cgtgggcttc tatggtgcgt tctacagcga tggcgagatc agtatctgca
      481 tggagcacat ggatggaggt tctctggatc aagtcctgaa gaaagctgga agaattcctg
      541 aacaaatttt aggaaaagtt agcattgctg taataaaagg cctgacatat ctgagggaga
      601 agcacaagat catgcacaga gatgtcaagc cctccaacat cctagtcaac tcccgtgggg
      661 agatcaagct ctgtgacttt ggggtcagcg ggcagctcat cgacgagatg gccaacgact
      721 tcgtgggcac aaggtcctac atgtcgccag aaagactcca ggggactcat tactctgtgc
      781 agtcagacat ctggagcatg
//
