LOCUS       sequence-54567-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Emrick MA, Lee T, Starkey PJ, Mumby MC, Resing KA, Ahn NG
  TITLE     The gatekeeper residue controls autoactivation of ERK2 via a pathway
            of intramolecular connectivity.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18101-6. Epub 2006 Nov
            17.
  PUBMED    17114285
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2006-11-28"; volume: "103(48)"; pages: 
            "18101-6. Epub 2006 Nov 17"
COMMENT     Sequence Label: pCMV5-rat ERK2-WT
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..22
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagaa ttctaaggag gtttaaccat ggcacatcac catcaccatc acgccatggc
       61 ggcggcggcg gcgggcccgg agatggtccg cgggcaggtg ttcgacgtgg ggccgcgcta
      121 cactaatctc tcgtacatcg gagaaggcgc ctacggcatg gtttgttctg cttatgataa
      181 tctcaacaaa gttcgagttg ctatcaagaa aatcagtcct tttgagcacc agacctactg
      241 tcagagaacc ctgagagaga taaaaatcct actgcgcttc agacatgaga acatcatcgg
      301 catcaatgac atcatccggg caccaaccat tgagcagatg aaagatgtat atatagtaca
      361 ggacctcatg gagacagatc tttacaagct cttgaagaca cagcacctca gcaatgatca
      421 tatctgctat tttctttatc agatcctgag aggattaaag tatatacatt cagctaatgt
      481 tctgcaccgt gacctcaagc cttccaacct cctgctgaac accacttgtg atctcaagat
      541 ctgtgacttt ggccttgccc gtgttgcaga tccagaccat gatcatacag ggttcttgac
      601 agagtatgta gccacgcgtt ggtacagagc tccagaaatt atgttgaatt ccaagggtta
      661 taccaagtcc attgatattt ggtctgtggg ctgcatcctg gcagagatgc tatccaacag
      721 gcctatcttc ccaggaaagc attaccttga ccagctgaat cacatcctgg gtat
//
