LOCUS       sequence-54577-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Emrick MA, Hoofnagle AN, Miller AS, Ten Eyck LF, Ahn NG
  TITLE     Constitutive activation of extracellular signal-regulated kinase 2 
            by synergistic point mutations.
  JOURNAL   J Biol Chem. 2001 Dec 7;276(49):46469-79. Epub 2001 Oct 8.
  PUBMED    11591711
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-12-7"; volume: "276(49)"; pages: "46469-79. Epub
            2001 Oct 8"
COMMENT     Sequence Label: pCMV5-rat ERK2-L73P
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..22
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagaa ttctaaggag gtttaaccat ggcacatcac catcaccatc acgccatggc
       61 ggcggcggcg gcgggcccgg agatggtccg cgggcaggtg ttcgacgtgg ggccgcgcta
      121 cactaatctc tcgtacatcg gagaaggcgc ctacggcatg gtttgttctg cttatgataa
      181 tctcaacaaa gttcgagttg ctatcaagaa aatcagtcct tttgagcacc agacctactg
      241 tcagagaacc ctgagagaga taaaaatccc actgcgcttc agacatgaga atattatcgg
      301 catcaatgac atcatccggg caccaaccat tgagcagatg aaagatgtat atatagtaca
      361 ggacctcatg gagacagatc tttacaagct cttgaagaca cagcacctca gcaatgatca
      421 tatctgctat tttctttatc agatcctgag aggattaaag tatatacatt cagctaatgt
      481 tctgcaccgt gacctcaagc cttccaacct cctgctgaac accacttgtg atctcaagat
      541 ctgtgacttt ggccttgccc gtgttgcaga tccagaccat gatcatacag ggttcttgac
      601 agagtatgta gccacgcgtt ggtacagagc tccagaaatt atgttgaatt cca
//
