LOCUS       sequence-54591-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Ptb
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        224..242
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     224..241
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     255..274
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             333..356
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
       61 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
      121 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac
      181 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
      241 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct
      301 cggttctatc gattgaattc accatggaaa tggactacaa ggatgacgat gacaagggtg
      361 gtggtggtgg tgcgtcgacg atggacggca tcgtcccaga catagcagtc ggtacaaagc
      421 ggggatccga cgagctcttc tccacgtgtg tcagcaacgg ccccttcatc atgagcagct
      481 ctgcctcagc agccaatgga aacgatagca agaagttcaa aggtgacaac aggagcgcag
      541 gagtcccttc cagagtcatc catgtcagaa agctgcccag cgatgtcact gagggcgagg
      601 tcatctccct agggctgccc tttggaaagg ttaccaacct tctcatgctg aaggggaaga
      661 accaggcctt cattgagatg aacacagagg aggctgccaa cactatggtt aactactata
      721 catcggtggc gccagtgctt cgtggacagc ccatctacat ccagttctcc aaccacaaag
      781 agctcaagac cgacagctcg cccaaccagg cacgtgccca ggcagccctg caggctgtaa
      841 actccgtcca gtctggaaac ctggccttgg cagcgtccgc tgctgccgtg gatgcaggaa
      901 tggcaatggg cagggcaga
//
