LOCUS       sequence-54616-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN backbone vector for use with the Golden Gate TALEN Kit in 
            place of pTAL1-4. Shorter N- and C-term tal protein segments 
            increases mutation induction; Fok I RR (improved version = 
            pCS2TAL3-RRR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS,
            Weis AM, Voytas DF, Grunwald DJ
  TITLE     Simple Methods for Generating and Detecting Locus-Specific Mutations
            Induced with TALENs in the Zebrafish Genome.
  JOURNAL   PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16.
  PUBMED    22916025
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012-08"; volume: "8(8)"; pages: "e1002861. Epub 
            2012 Aug 16"
COMMENT     Sequence Label: pCS2TAL3-RR
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..614)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQAREMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    800..821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(836..841,842..859,860..866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    874..890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atacgactca ctatagttct agattaaaag tttatctcgc cgttattaaa tttccggcgc
       61 acttcctcta aggttaatgt gccggcttta atcatttctc caccaattaa aagctcttct
      121 acactaagaa cagctccatt acaattagtg atatgattta atcgtgtaag ctgagctttg
      181 tagtttcctt taaagtgacc actcacaaat aaaaacttaa attccgttac agaagatgga
      241 tagactttcc accattcatt agggttgata tgtttgtttc gtgtttgatt ttcttcgaca
      301 tatcgttgca tttcacgtgc ttggccaatt ggcagattat aacctccgct ataagcttta
      361 gtatccacga tcacaccgta atcaatagga gatccgacag tataaattgc tccgtccggt
      421 ttccttgatc cacccaaatg tttacctcta tatccataaa ctttcataaa aaattccatt
      481 accttcattt caagaattct atcctgagtg gaatttctgg caatttcaat taattcaata
      541 tattcatgag gcacatattt caatttatga cgaagttcag atttcttctc ctccagttca
      601 cttttgacta gttgggatcc ggcaacgcga tgggacgtgc gttcgccaat acggcgattg
      661 actcttctga tcaattccgg cgcgtgcggc aatccctttt tcactgcatc catggcagga
      721 cgtccgccga ggcaggccaa ggcgacgagg tggtcgttgg agacgaccgg tggaaagcgg
      781 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      841 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttccc
//
