LOCUS       sequence-54644-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T
  TITLE     Translation elongation can control translation initiation on 
            eukaryotic mRNAs.
  JOURNAL   EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 
            2013 Dec 19.
  PUBMED    24357599
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/embj.201385651"; journalName: "EMBO J"; date: "2014-01-1- 
            1"; volume: "33"; issue: "1"; pages: "21-34"
COMMENT     Sequence Label: pTH736-CEN-staHIS3
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      174..473
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        complement(521..539)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(560..576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(573..595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(608..614,615..632,633..638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    653..674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(791..808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtttgctgtc ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg
       61 tcattgttct cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac
      121 caagctacaa tcaactcccc gggtaccgag ctcgaattcg acactctaaa taagcggatc
      181 tcttatgtct ttacgattta tagttttcat tatcaagtat gcctatatta gtatatagca
      241 tctttagatg acagtgttcg aagtttcacg aataaaagat aatattctac tttttgctcc
      301 caccgcgttt gctagcacga gtgaacacca tccctcgcct gtgagttgta cccattcctc
      361 taaactgtag acatggtagc ttcagcagtg ttcgttatgt acggcatcct ccaacaaaca
      421 gtcggttata gtttgtcctg ctcctctgaa tcgtctccct cgatatttct cattttcctt
      481 cgcatgccct cgaggggggg cccggtacca gcttttgttc cctttagtga gggttaattt
      541 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      721 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      841 cagctcactc aaaggcggta at
//
