LOCUS       sequence-55126-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2 with H134R mutation fused to EYFP 
            driven by human Synapsin I promoter for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     hChR2 plasmids
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-hSyn-hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(35..958)
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="DYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGTN
                     GAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKNP
                     SMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWGA
                     TSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVSW
                     GMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDIR
                     KTTKLNIGGTEIEVETLVEDEAEAGAV"
ORIGIN
        1 cctcgccctt gctcaccatg gtggcggccg ctggcacggc tccggcctcg gcttcgtctt
       61 cgacgagagt ctcgacctcg atctccgttc cgccgatgtt cagtttggtg gttttgcgga
      121 tatctccgtg aatcaatatg tgctcgtgga tcaggacgcg caggtagtgt cccaacaacc
      181 cccaacaatt tttactcatc agatcaataa tcgtgtgacc tacggtggag ccatagacgc
      241 tcaggacgcc aaaaccttcg ggccccaaaa tgaagagaat tgggaacata ccccagctca
      301 cgaaaaacag ccatgccatg ccggtcacga cctggcggca ccgacccttt ggcacagtat
      361 gataaccctc gatatatgct ttggcggcgt gaaaaaatgt gttcgcgcca tagcacaatc
      421 caagacaaaa gaagatgact ttaacatagc cggttgccat ggcgctggta gccccccaca
      481 cgatagtccc gatgtctgag acaaggagtc ccatggttct cctgctgtag tcgttgctca
      541 ggccggtgag gttgctcagg cggataagga tgacaggaca agtgagcagc cactctgcat
      601 agcgcagcca ctgcacccgg tgtcctgtgg caaggtagag catagaggga ttcttaaact
      661 caaaaaagaa ctcgagaatc accttaacca tttcaatggc gcacacatag atctcctccc
      721 agccgcatgt agatttccag gtttggtagg catagaacat cagcagcaaa atgctgaatc
      781 ctgctgcaag ccactgcagg acatttgacg cggtctgagc gccgttcgtg ccgcgagatt
      841 caatccatcc ggcacagtaa cattgatcct cagggaccag gacggacccg ttcaccacca
      901 caggattagt aacgaacaaa agttcgcgtc cgacggcaga caaagcgccg ccatagtcca
//
