LOCUS       sequence-55128-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2 with H134R mutation fused to EYFP 
            driven by human Synapsin I promoter for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     hChR2 plasmids
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-hSyn-hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      434..443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(481..928)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 ctgtagtcgt tgctcaggcc ggtgaggttg ctcaggcgga taaggatgac aggacaagtg
       61 agcagccact ctgcatagcg cagccactgc acccggtgtc ctgtggcaag gtagagcata
      121 gagggattct taaactcaaa aaagaactcg agaatcacct taaccatttc aatggcgcac
      181 acatagatct cctcccagcc gcatgtagat ttccaggttt ggtaggcata gaacatcagc
      241 agcaaaatgc tgaatcctgc tgcaagccac tgcaggacat ttgacgcggt ctgagcgccg
      301 ttcgtgccgc gagattcaat ccatccggca cagtaacatt gatcctcagg gaccaggacg
      361 gacccgttca ccaccacagg attagtaacg aacaaaagtt cgcgtccgac ggcagacaaa
      421 gcgccgccat agtccatggt ggctccggag tcgactctag aggatccggt accttctcga
      481 ctgcgctctc aggcacgaca cgactcctcc gctgcccacc gcagactgag gcagcgctga
      541 gtcgccggcg ccgcagcgca gatggtcgcg cccgtgcccc cctatctcgc gcctcgcgtg
      601 gtgcggtccg gctgggccgg cggcggcgcg gacgcgacca aggtggccgg gaaggggagt
      661 ttgcggggga ccggcgagtg acgtcagcgc gccttcagtg ctgaggcggc ggtggcgcgc
      721 gccgccaggc gggggcgaag gcactgtccg cggtgctgaa gctggcagtg cgcacgcgcc
      781 tcgccgcatc ctgtttcccc tccccctctc tgatagggga tgcgcaattt ggggaatggg
      841 ggttgggtgc ttgtccagtg ggtcggggtc ggtcgtcagg taggcacccc caccccgcct
      901 catcctggtc ctaaaaccca cttgcactca tacgcagggc cctctgcagt ctagaca
//
