LOCUS       sequence-55143-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Wright DJ, King K, Modrich P
  TITLE     The negative charge of Glu-111 is required to activate the cleavage 
            center of EcoRI endonuclease.
  JOURNAL   J Biol Chem. 1989 Jul 15;264(20):11816-21.
  PUBMED    2745418
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1989-07-15"; volume: "264(20)"; pages: "11816-21"
COMMENT     Sequence Label: pEQ111m
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..28
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             187..252
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
ORIGIN
        1 ctcttaaaat taagccctga agaagggcag cattcaaagc agaaggcttt ggggtgtgtg
       61 atacgaaacg aagcattggc cgtaagtgcg attccggatt agctgccaat gtgccaatcg
      121 cggggggttt tcgttcagga ctacaactgc cacacaccac caaagctaac tgacaggaga
      181 atccagatgg atgcacaaac acgccgccgc gaacgtcgcg cagagaaaca ggctcaatgg
      241 aaagcagcaa atcccctgtt ggttggggta agcgcaaaac cagttccgga tccggctgga
      301 aatacaggaa cgcacactgg atggtccttc gttgtcgctg tgatggcgaa actatgaaaa
      361 atggcaggtt cggtggattt tgacgggcta atgtggtctg caccatctgg ttgcataggt
      421 attcatacgg ttaaaattta tcaggcgcga tcgcggcagt ttttcgggtg gtttgttgcc
      481 atttttacct gtctgctgcc gtgatcgcga tgaacgcgtt ttagcggtgc gtacaattaa
      541 gggattatgg taaatcaaac gtatgttaat ctatcgacat atgtaacttt ataaaataac
      601 agtggaaaca tggattcatg tctaataaaa aacagtcaaa taggctaact gaacaacata
      661 agttatctca aggtgtaatt gggatttttg gggattatgc aaaagctcat gatctcgctg
      721 ttggtgaggt ttcaaaatta gtaaagaaag ctcttagcaa cgaataccct caattatcat
      781 ttcgatatag agatagtata aagaaaacag aaataaatga agctttaaaa
//
