LOCUS       sequence-55148-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral vector for reversible immortalization of 
            human primary cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-TERT-iresTK
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    190..223
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
       61 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
      121 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
      181 aaaagcagca taacttcgta taatgtatgc tatacgaagt tattcccatg gtgaattgat
      241 cccatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa
      301 gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg tcgaggaatt
      361 cggtacccct tccgtgtttc agttagcctc ccccatctcc cgggcaaacg tgcgcgccag
      421 gtcgcagatc gtcggtatgg agccgggggt ggtgacgtgg gtctggacca tcccggaggt
      481 aagttgcagc agggcgtccc ggcagccggc gggcgattgg tcgtaatcca ggataaagac
      541 gtgcatggga cggaggcgtt tggccaagac gtccaaggcc caggcaaaca cgttgtacag
      601 gtcgccgttg ggggccagca actcgggggc ccgaaacagg gtaaataacg tgtccccgat
      661 atggggtcgt gggcccgcgt tgctctgggg ctcggcaccc tggggcggca cggccgtccc
      721 cgaaagctgt ccccaatcct cccgccacga cccgccgccc tgcagatacc gcaccgtatt
      781 ggcaagcagc ccgtaaacgc ggcgaatcgc ggccagcata gccaggtcaa gccgctcgcc
      841 ggggcgctgg cgtttggcca ggcggtcgat gtgtctgtcc tccggaaggg cccccaacac
      901 gatgtttgtg ccgggcaagg tcggcgggat gagggccacg aacgccagca cggcctgggg
      961 ggtcatgctg cc
//
