LOCUS       sequence-55151-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Unpublished
  TITLE     Jonathon Pines lab pcDNA5/FRT/TO-based cloning vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA5/FRT/TO-neo (1795)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(33..80)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     212..231
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    364..588
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     577..594
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(790..814)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    824..842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    845..863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatggcca aggaagttcc tatactttct agagaatagg
       61 aacttcggaa taggaacttc taggtacgtg aaccatcacc ctaatcaagt tttttggggt
      121 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
      181 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
      241 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
      301 cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag
      361 aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct
      421 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
      481 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
      541 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
      601 agcgggttta aacgggccct ctagactcga gcggccgcca ctgtgctgga tatctgcaga
      661 attccaccac actggactag tggatccgag ctcggtacca agcttaagtt taaacgctag
      721 agtccggagg ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg
      781 tctccaggcg atctgacggt tcactaaacg agctcgtcga cgatctctat cactgatagg
      841 gagatctcta tcactgatag ggagagctct gcttatatag acctcc
//
