LOCUS       sequence-55169-e        9394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression, TRE-gateway-HA; PGK-rtTA-2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9394)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX_402
FEATURES             Location/Qualifiers
     source          1..9394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    555..679
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(704..709,710..727,728..734)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             788..1447
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(854..873)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1789..2094
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1973..1992
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2135..2259)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2267..2293
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2309..2326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2336..2846
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2765..2783
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2856..3452
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2856..2875)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3352..3372
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3453..3506
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3507..4124,4125..4130,4131..4166,4167..4169,4170..4205,
                     4206..4208,4209..4244,4245..4253)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4293..4313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4711..4722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4927..5160
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5232..5353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5269..5288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5323..5342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5393..5528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5528..5599
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5600..5668,5669..6460)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5818..5837)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6631..7219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7120..7139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7373..7390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7507..7528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7543..7548,7549..7566,7567..7573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7581..7597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7586..7608
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7605..7621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7605..7621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7640..7660
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7642..7660
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7688..7914
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7915..8095
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8142..8267
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8760..8993
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9178..9222
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcgattg atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
      601 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
      661 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc
      721 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
      781 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      841 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      901 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      961 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     1021 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     1081 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     1141 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     1201 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     1261 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     1321 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     1381 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     1441 ggcgtaaaga tctggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     1501 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     1561 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     1621 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     1681 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     1741 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     1801 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     1861 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     1921 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     1981 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     2041 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     2101 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     2161 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     2221 attttacgtt tctcgttcag ctttcttgta caaagtggtt accggttatc cgtatgatgt
     2281 gccggattat gcgtaaacgc gtccggtcca ccaccaccac caccactaag gatccggggt
     2341 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     2401 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     2461 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     2521 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     2581 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     2641 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     2701 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     2761 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     2821 accgaatcac cgacctctct ccccagcaat tcaccatgac cgagtacaag cccacggtgc
     2881 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2941 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     3001 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     3061 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     3121 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     3181 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     3241 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     3301 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     3361 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     3421 gcacctggtg catgacccgc aagcccggtg ccgaaggtag aggttctctc ctcacttgtg
     3481 gtgatgttga agaaaaccct ggtccaatgt ctagactgga caagagcaaa gtcataaacg
     3541 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     3601 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     3661 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     3721 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     3781 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     3841 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     3901 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     3961 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     4021 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     4081 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     4141 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     4201 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     4261 aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct atgtggatac
     4321 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4381 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4441 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4501 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4561 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4621 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4681 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4741 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4801 cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac
     4861 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4921 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4981 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5041 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5101 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5161 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5221 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5281 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5341 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     5401 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5461 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5521 tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5581 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5641 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5701 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5761 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5821 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5881 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5941 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6001 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6061 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6121 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6181 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6241 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6301 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6361 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6421 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7321 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7381 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7441 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7501 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7561 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7621 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     7681 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     7741 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     7801 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     7861 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     7921 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7981 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8041 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     8101 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     8161 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     8221 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     8281 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     8341 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     8401 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     8461 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     8521 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     8581 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     8641 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     8701 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     8761 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     8821 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     8881 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8941 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     9001 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     9061 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     9121 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     9181 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     9241 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     9301 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     9361 tcgtttcaga cccacctccc aaccccgagg ggac
//
