LOCUS       sequence-55177-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T
  TITLE     Translation elongation can control translation initiation on 
            eukaryotic mRNAs.
  JOURNAL   EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 
            2013 Dec 19.
  PUBMED    24357599
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/embj.201385651"; journalName: "EMBO J"; date: "2014-01-1- 
            1"; volume: "33"; issue: "1"; pages: "21-34"
COMMENT     Sequence Label: pTH736-CEN-staHIS3
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(192..218)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(219..878)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 aataataaaa atcataaatc ataagaaatt cgcttattta gaagtgtcaa caacgtatct
       61 accaacgatt tgaccctttt ccatcttttc gtaaatttct ggcaaggtag acaagccgac
      121 aaccttgatt ggagacttga ccaaacctct ggcgaagaag tccaaagctt gcatgcctgc
      181 aggtcgacct acgcgtagtc cgggacgtcg tacgggtaca taagaacacc tttggtggag
      241 ggaacatcgt tggtaccatt gggcgaggtg gcttctctta tggcaaccgc aagagccttg
      301 aacgcactct cactacggtg atgatcattc ttgcctcgca gacaatcaac gtggagggta
      361 attctgctag cctctgcaaa gctttcaaga aaatgcggga tcatctcgca agagagatct
      421 cctactttct ccctttgcaa accaagttcg acaactgcgt acggcctgtt cgaaagatct
      481 accaccgctc tggaaagtgc ctcatccaaa ggcgcaaatc ctgatccaaa cctttttact
      541 ccacgcacgg cccctagggc ctctttaaaa gcttgaccga gagcaatccc gcagtcttca
      601 gtggtgtgat ggtcgtctat gtgtaagtca ccaatgcact caacgattag cgaccagccg
      661 gaatgcttgg ccagagcatg tatcatatgg tccagaaacc ctatacctgt gtggacgtta
      721 atcacttgcg attgtgtggc ctgttctgct actgcttctg cctctttttc tgggaagatc
      781 gagtgctcta tcgctagggg accacccttt aaagagatcg caatctgaat cttggtttca
      841 tttgtaatac gctttactag ggctttctgc tctgtcatg
//
