LOCUS       sequence-55233-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72113-BBa_J72152
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(214..257)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
      121 aaagttggaa cctcttacgt gcccgatcaa gaattgccat tatcaacaag tttgtacaaa
      181 aaagcaggct ccgaattggt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
      241 tcgctaagga tgatttctgg aattcatgag atctctatgc tactccatcg agccgtcaat
      301 tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca
      361 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
      421 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
      481 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
      541 taactgctgg cggaaaagac gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
      601 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
      661 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagcaa
      721 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
      781 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
      841 gaaccccgta ttggcaatat gacgcagta
//
