LOCUS       sequence-55234-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72113-BBa_J72103
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    163..187
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(211..254)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
       61 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
      121 gttggaacct cttacgtgcc cgatcaagaa ttgccattat caacaagttt gtacaaaaaa
      181 gcaggctccg aattggtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
      241 ctaaggatga tttctggaat tcatgagatc tctatgctac tccatcgagc cgtcaattgt
      301 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
      361 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
      421 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
      481 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
      541 ctgctggcgg aaaagacgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
      601 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
      661 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagcaacaa
      721 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
      781 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
      841 ccccgtatgc aatatg
//
