LOCUS       sequence-55389-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Zhao KW, Sikriwal D, Dong X, Guo P, Sun X, Dong JT
  TITLE     Oestrogen causes degradation of KLF5 by inducing the E3 ubiquitin 
            ligase EFP in ER-positive breast cancer cells.
  JOURNAL   Biochem J. 2011 Jul 15;437(2):323-33.
  PUBMED    21542805
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "2011-07-15"; volume: "437(2)"; pages: "323-33"
COMMENT     Sequence Label: pcDNA3-HA-KLF5-his
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccgccac catggactat ccttatgatg ttcctgatta
      121 tgcagaattc atggctacaa gggtgctgag catgagcgcc cgcctgggac ccgtgcccca
      181 gccgccggcg ccgcaggacg agccggtgtt cgcgcagctc aagccggtgc tgggcgccgc
      241 gaatccggcc cgcgacgcgg cgctcttccc cggcgaggag ctgaagcacg cgcaccaccg
      301 cccgcaggcg cagcccgcgc ccgcgcaggc cccgcagccg gcccagccgc ccgccaccgg
      361 cccgcggctg cctccagagg acctggtcca gacaagatgt gaaatggaga agtatctgac
      421 acctcagctt cctccagttc ctataattcc agagcataaa aagtatagac gagacagtgc
      481 ctcagtcgta gaccagttct tcactgacac tgaagggtta ccttacagta tcaacatgaa
      541 cgtcttcctc cctgacatca ctcacctgag aactggcctc tacaaatccc agagaccgtg
      601 cgtaacacac atcaagacag aacctgttgc cattttcagc caccagagtg aaacgactgc
      661 ccctcctccg gccccgaccc aggccctccc tgagttcacc agtatattca gctcacacca
      721 gaccgcagct ccagaggtga acaatatttt catcaaacaa gaacttccta caccagatct
      781 tcatctttct gtccctaccc agcagggcca cctgtaccag ctactgaata caccggatct
      841 agatatgccc agtt
//
