LOCUS       sequence-55487-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     Kuwabara unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPK719
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..58
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     67..83
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     67..83
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aatttaaata tgtatggtta gttagaagtt cctattcttc aaatagtata ggaacttcgc
       61 ggccgctcta gaactagtgg atcctcagca ctgtcctgct ccttgtgatg gtttacaaac
      121 gtaaaaagtc tctttaatac ctgtttttgc ttcatattgt tcagcgacag cttgctgtac
      181 ggcaggcacc agctcttccg ggatcagcgc gacgatacag ccgccaaatc cgccgccggt
      241 catgcgtacg ccacctttgt cgccaatcac agctttgacg atttctacca gagtgtcaat
      301 ttgcggcacg gtgatttcga aatcatcgcg catagaggca tgagactccg ccatcaactc
      361 gcccatacgt ttcaggtcgc cttgctccag cgcgctggca gcttcaacgg tgcgggcgtt
      421 ttcagtcagt atatgacgca cgcgttttgt cacgatcggg tccagttcat gcgcaacagc
      481 gttgaactct tcaatggtga catcacgcag ggctggctgc tggaagaaac gcgcaccggt
      541 ttcgcactgt tcacgacggg tgttgtattc gctaccaacc agggtacgtt tgaagttact
      601 gttgatgatg acgacagcca cacctttggg catggaaact gctttggtcc ccagtgagcg
      661 gcaatcgatc agcaaggcat gatctttctt gccgagcgcg gaaattagct gatccatgat
      721 cccgcagtta cagcctacaa actggttttc tgcttcctga ccgttaagcg cgatttgtgc
      781 gccgtccagc ggcagatgat aaagctgctg caatacggtt ccgaccgcga cttccagtga
      841 agcggaagaa cttaacccgg caccctgcgg cacattgccg ctgatcacca tgtccacgcc
      901 gccgaagctg ttgttacgca gttgcagatg tttcac
//
