LOCUS       sequence-55529-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Yan X, Habedanck R, Nigg EA
  TITLE     A complex of two centrosomal proteins, CAP350 and FOP, cooperates 
            with EB1 in microtubule anchoring.
  JOURNAL   Mol Biol Cell. 2006 Feb;17(2):634-44. Epub 2005 Nov 28.
  PUBMED    16314388
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2006-02"; volume: "17(2)"; pages: "634-44. Epub 2005 
            Nov 28"
COMMENT     Sequence Label: pcDNA3 CAP350 (Nigg XY97)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..117
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             121..150
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             154..183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             187..210
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct taccatggag cagaagctga tctccgagga ggacctgaac
      121 gagcagaagc tgatctccga ggaggacctg aacgagcaga agctgatctc cgaggaggac
      181 ctgaacctgg aggtgctgtt ccagggaccc ggggtaccga tgaggagcag caaatcaaaa
      241 gaggtgcctt taccaaatcc aaggaactct caaagcaagg atactgttca agcagatata
      301 accacatcgt gggatgcact ttctcaaacc aaggctgctc tgagacacat tgaaaataaa
      361 ttagaagtag cccctacaag tacagctgtg tgtgattctg tcatggatac caagaagtct
      421 tctacaagtg ctactcgaaa aataagtaga aaagatggta gatacctgga tgattcttgg
      481 gttaatgctc caatctccaa atccactaaa tcacgaaaag agaaatctcg tagtcctctc
      541 agggccacca ccctggagag taatgtgaag aaaaataatc gtgtggaatt tcgtgaacct
      601 ttggtttctt atagggaaat ccatggtgca ccttccaatt tcagttccag ccatctggaa
      661 tcaaagcacg tatactgtgt agatgttaat gaagaaaaga ctgagagcgg taactggatg
      721 ataggcagtc gagaagaacg gaatatacgg agctgtgatt ttgagagctc ccaatcatct
      781 gtcatcaatg atacagttgt taggttttta aatgatcgac cagcaattga tgcattgcaa
      841 aattctgaat gtt
//
