LOCUS       sequence-55531-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Chan EH, Nousiainen M, Chalamalasetty RB, Schafer A, Nigg EA, Sillje
            HH
  TITLE     The Ste20-like kinase Mst2 activates the human large tumor 
            suppressor kinase Lats1.
  JOURNAL   Oncogene. 2005 Mar 17;24(12):2076-86.
  PUBMED    15688006
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2005-03-17"; volume: "24(12)"; pages: "2076-86"
COMMENT     Sequence Label: pcDNA3 Lats1 (Nigg HS189)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             129..158
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             162..191
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             195..218
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta ccatggagca gaagctgatc tccgaggagg
      121 acctgaacga gcagaagctg atctccgagg aggacctgaa cgagcagaag ctgatctccg
      181 aggaggacct gaacctggag gtgctgttcc agggacccgg ggtaccagga tctaccatga
      241 agaggagtga aaagccagaa ggatatagac aaatgaggcc taagaccttt cctgccagta
      301 actatactgt cagtagccgg caaatgttac aagaaattcg ggaatccctt aggaatttat
      361 ctaaaccatc tgatgctgct aaggctgagc ataacatgag taaaatgtca accgaagatc
      421 ctcgacaagt cagaaatcca cccaaatttg ggacgcatca taaagccttg caggaaattc
      481 gaaactctct gcttccattt gcaaatgaaa caaattcttc tcggagtact tcagaagtta
      541 atccacaaat gcttcaagac ttgcaagctg ctggatttga tgaggatatg gttatacaag
      601 ctcttcagaa aactaacaac agaagtatag aagcagcaat tgaattcatt agtaaaatga
      661 gttaccaaga tcctcgacga gagcagatgg ctgcagcagc tgccagacct attaatgcca
      721 gcatgaaacc agggaatgtg cagcaatcag ttaaccgcaa acagagctgg aaaggttcta
      781 aagaatcctt agttcctcag aggcatggcc cgccactagg agaa
//
