LOCUS       sequence-55600-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Brumbaugh J, Hou Z, Russell JD, Howden SE, Yu P, Ledvina AR, Coon 
            JJ, Thomson JA
  TITLE     Phosphorylation regulates human OCT4.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 
            2.
  PUBMED    22474382
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-05-8"; volume: "109(19)"; pages: 
            "7162-8. Epub 2012 Apr 2"
COMMENT     Sequence Label: pGEX4T-1_OCT4_T234A
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             654..674
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             681..698
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(751..769)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      765..812
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaagacacc ctgggatata cacaggccga tgtggggctc accctggggg ttctatttgg
       61 gaaggtattc agccaaacga ccatctgccg ctttgaggct ctgcagctta gcttcaagaa
      121 catgtgtaag ctgcggccct tgctgcagaa gtgggtggag gaagctgaca acaatgaaaa
      181 tcttcaggag atatgcaaag cagaaaccct cgtgcaggcc cgaaagagaa agcgagccag
      241 tatcgagaac cgagtgagag gcaacctgga gaatttgttc ctgcagtgcc cgaaacccac
      301 actgcagcag atcagccaca tcgcccagca gcttgggctc gagaaggatg tggtccgagt
      361 gtggttctgt aaccggcgcc agaagggcaa gcgatcaagc agcgactatg cacaacgaga
      421 ggattttgag gctgctgggt ctcctttctc agggggacca gtgtcctttc ctctggcccc
      481 agggccccat tttggtaccc caggctatgg gagccctcac ttcactgcac tgtactcctc
      541 ggtccctttc cctgaggggg aagcctttcc ccctgtctcc gtcaccactc tgggctctcc
      601 catgcattca aactgagaat tcgagctccg tcgacaagct tacggccgca ctcgagaacc
      661 tgtacttcca gggagtcgag caccaccacc accaccactg agatccggct gctaacaaag
      721 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      781 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      841 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      901 cgtgaccgct acacttgcca gcgc
//
