LOCUS       sequence-55620-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SETD2
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             958..975
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcgaa acgagcgttc cgccgggtag
      121 tgcactggtt gggccgagtt gtgtgatgga cgatttccgc gacccgcaac gttggaagga
      181 atgtgctaaa cagggcaaaa tgccgtgcta ttttgatctg atcgaagaga acgtctacct
      241 gactgagcgc aagaaaaaca aatcacaccg tgatatcaag cgcatgcaat gtgaatgcac
      301 accgctgtcc aaagacgaac gtgcgcaggg tgaaatcgcg tgtggggaag attgtctgaa
      361 tcgcctgctg atgatcgagt gttcgagccg ttgcccgaac ggtgactatt gttctaatcg
      421 ccgtttccag cgcaaacaac acgcagacgt tgaagtcatt ctgacagaga agaaagggtg
      481 gggcctgcgc gctgcaaagg atctgccgag taacacgttt gtgctggagt actgcggtga
      541 agtactggac cacaaggagt tcaaagcccg cgttaaagag tatgcacgta ataagaacat
      601 tcactattac tttatggctc tgaaaaatga tgaaattatc gacgcgactc agaaaggcaa
      661 ctgttcacgc ttcatgaatc actcctgcga gccgaactgt gaaacacaaa agtggaccgt
      721 gaatggtcag ctgcgtgtcg ggttttttac gaccaagctg gtcccgtctg ggtcggaact
      781 gactttcgat taccaattcc agcgctatgg taaagaggcc cagaagtgct tctgtgggag
      841 tgcgaactgc cgtggctatc tggggggcga gaaccgcgta agtattcgtg cggccggtgg
      901 gaaaatgaag aaggagcgct cacgtaaatg atgacgaagc ttgcggccgc actcgagcac
      961 caccaccacc accactga
//
