LOCUS       sequence-55629-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pEF5/FRT-DEST EVC2-Flag
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(131..154)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact
       61 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtctagata
      121 tctcgactca cttgtcatcg tcgtccttgt aatctccgcc agatcctggt gttccgccac
      181 gcgtgtccaa gcccaaggcc ctgttggcct tcttggcatt caggaaattc tttttcctcc
      241 tgggaggaac acgcaaagag atctcgggct cccttgggct tctgaataca aagatctttt
      301 ctcccgtgtt taatatgtcc atagcgtctg ccccagtgat ggggatggga gccagttctc
      361 caatgggttc tagggacagg tgtggccagc ttcctttccc aggaaacatc accctctcct
      421 ggacgggaac atatatcttc ttcaatatgc gctccttctt ctggcgggcc cagagcatcc
      481 tctccagtcc ttgactcacc agatctgctc tgaatctgct gtccaaaacc ttccaccaaa
      541 cttggtgttt cctcctcttg ctctgctctg cctgagcctg ctcaccactg ctctcattct
      601 cagctgtgtg gtcggagtgc ttctcactga ctgcgtccag cagggccagt agctgaggct
      661 gtgaagccgc tggaagcacc acactcagga gtcgcagtag tgtgctgcct ggtaccattg
      721 tcatccttga gaggtatgac accagcctca gtccctggct gcaaaggctg gccatggtgt
      781 cagcttcaat gctttccaga
//
