LOCUS       sequence-55730-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_306
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(154..278)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(299..799)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(362..380)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcagc ttttttgtac aaacttgttg atcaatcgat gctagcccct
      301 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
      361 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
      421 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
      481 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
      541 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
      601 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
      661 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
      721 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
      781 tgccttggaa aaggcgcaac ctgcaggcct cgagccggcg ccaaagtgga tctctgctgt
      841 ccctgtaata aacc
//
