LOCUS       sequence-55816-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA
  TITLE     Sequence- and structure-specific RNA processing by a CRISPR 
            endonuclease.
  JOURNAL   Science. 2010 Sep 10;329(5997):1355-8.
  PUBMED    20829488
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-09-10"; volume: "329(5997)"; pages: "1355-8"
COMMENT     Sequence Label: pHMGWA-Pa14Csy4H29A
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(86..649)
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLAQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     CDS             complement(659..679)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(700..724)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(746..769)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgaggtacat caccactttg tacaagaaag
       61 ctgggtcggc gcgcccaccc ttttatcaga accagggaac gaaacctcct ttgctcaacc
      121 cgtaacaggt gaatcctcct tcctctgccg tcacctgcaa cggcccgtgg cggatgaaga
      181 gacggaagtg ctgtccggtg ctctggctgc gtagcgtgac gaagggcagg tccaaggctc
      241 tcgcgaccgt atcgggaatg cgtttccgag cctcctcctc actcagatcg tgccggcgca
      301 tgagccgccg ccgcaggcgt tccggattgc ttttcgcctg aacccgactg acctgacggt
      361 acggtgtggg gtgaggcacg actgccggtt ctccgaattg cagatggtcc cgcaaccctt
      421 ccagccaggg ccgggcgagc agggcacgaa ggtcgtccgc cgaggcatga atgcgcaggc
      481 gctcgcccag ccgggagcgg ctttcgtcga ggtcggggaa gctcacgccg atcctgtccc
      541 cgccctgtgc caccagggcc tgggcgagct tgccgaagag cacgctcatg agttgcgccg
      601 ggggaaattc cgggtccggt cgcaagcgaa tgtcgaggta gtggtccatg gtgaaggagc
      661 cctgaaaata caggttttcc tcgagtacgg cggccgcgga gcctgctttt ttgtacaaac
      721 ttgtgatgta agatccggta ccagtctgcg cgtctttcag ggcttcatcg acagtctgac
      781 gaccgctgg
//
