LOCUS       sequence-55859-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Fukunaga R, Han BW, Hung JH, Xu J, Weng Z, Zamore PD
  TITLE     Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and
            Mammals.
  JOURNAL   Cell. 2012 Oct 9. pii: S0092-8674(12)01171-3. doi: 
            10.1016/j.cell.2012.09.027.
  PUBMED    23063653
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2012 
            Oct 9. pii: S0092-8674(12)01171-3. doi: 10.1016/j.cell.2012.09.027."
COMMENT     Sequence Label: pcold-6xHis-HRV3Csite-HsTRBP
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..17
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..47
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             84..107
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ccatgaatca caaagtgcat catcatcatc atcatatcga aggtaggcat atggagctcg
       61 gtaccctcga gggatccgaa ttccttgaag tcctctttca gggacccgtc gacatgagtg
      121 aagaggagca aggctccggc actaccacgg gctgcgggct gcctagtata gagcaaatgc
      181 tggccgccaa cccaggcaag accccgatca gccttctgca ggagtatggg accagaatag
      241 ggaagacgcc tgtgtacgac cttctcaaag ccgagggcca agcccaccag cctaatttca
      301 ccttccgggt caccgttggc gacaccagct gcactggtca gggccccagc aagaaggcag
      361 ccaagcacaa ggcagctgag gtggccctca aacacctcaa aggggggagc atgctggagc
      421 cggccctgga ggacagcagt tctttttctc ccctagactc ttcactgcct gaggacattc
      481 cggtttttac tgctgcagca gctgctaccc cagttccatc tgtagtccta accaggagcc
      541 cccccatgga actgcagccc cctgtctccc ctcagcagtc tgagtgcaac cccgttggtg
      601 ctctgcagga gctggtggtg cagaaaggct ggcggttgcc ggagtacaca gtgacccagg
      661 agtctgggcc agcccaccgc aaagaattca ccatgacctg tcgagtggag cgtttcattg
      721 agattgggag tggcacttcc aaaaaattgg caaagcggaa tgcggcggcc aaaatgctgc
      781 ttcgagtgca cacggtgcct ctggatgccc gggatggcaa tgaggtggag cctgatgatg
      841 accacttctc cattggtgtg ggctcccgcc tggatggtct tcgaaaccgg ggcccaggtt
      901 gcacctggga ttctctacg
//
