LOCUS       sequence-55943-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Blakeley BD, Chapman AM, McNaughton BR
  TITLE     Split-superpositive GFP reassembly is a fast, efficient, and robust 
            method for detecting protein-protein interactions in vivo.
  JOURNAL   Mol Biosyst. 2012 Aug;8(8):2036-40. Epub 2012 Jun 12.
  PUBMED    22692102
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biosyst."; date: "2012-08"; volume: "8(8)"; pages: "2036-40. Epub 
            2012 Jun 12"
COMMENT     Sequence Label: pMRBad-Z-CspGFP
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             414..436
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(463..747)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(521..540)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ccttagttgt acattacttg tagcgttcgt
       61 cgcgtccgtg cttaatgcct gcagcggtca cgaattctaa cagaaccatg tgatcgcgtt
      121 tttctttcgg atctttgctc agtttagaac gggtgctcag gtagtggttg cgaggtaaca
      181 gtacaggccc gcgaccaatc ggcgtgtttt gttgataatg atccgctaat tgaacgctgc
      241 cgtctttcac gttatggcga attttgaatt ttgccttgat accattctta ccgcttccac
      301 ccgacgtctg cgcgagtttt ttttccaatg cttggttttt ccattccagc tgagcaagtt
      361 ttttctccag ggcttgtaac ttcttttcca gctgctcgct tgccatgggt atatctcctt
      421 cttaaagtta aacaaaatta tttctagccc aaaaaaacgg gtatggagaa acagtagaga
      481 gttgcgataa aaagcgtcag gtaggatccg ctaatcttat ggataaaaat gctatggcat
      541 agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt tctgccgtga ttatagacac
      601 ttttgttacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg cttttaataa
      661 gcggggttac cggtttggtt agcgagaaga gccagtaaaa gacgcagtga cggcaatgtc
      721 tgatgcaata tggacaattg gtttcttctc tgaatggcgg gagtatgaaa agtatggctg
      781 aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa tgcccatctg gtggcgggtt
      841 taacgccgat tgaggccaac ggttatctcg atttttttat cgaccgaccg ctgggaatga
      901 aaggttatat tctcaatctc accattcgcg gtcagggggt ggt
//
