LOCUS       sequence-56123-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Parikh MR, Greene DN, Woods KK, Matsumura I
  TITLE     Directed evolution of RuBisCO hypermorphs through genetic selection 
            in engineered E.coli.
  JOURNAL   Protein Eng Des Sel. 2006 Mar;19(3):113-9. Epub 2006 Jan 19.
  PUBMED    16423843
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng
            Des Sel."; date: "2006-03"; volume: "19(3)"; pages: "113-9. Epub 
            2006 Jan 19"
COMMENT     Sequence Label: rbcLS-pMAL-2px
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        662..739
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(759..778)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt
       61 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
      121 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
      181 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
      241 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
      301 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
      361 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
      421 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
      481 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
      541 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
      601 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgaaattc
      661 cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
      721 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
      781 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
      841 gcgg
//
