LOCUS       sequence-56230-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo single-strand annealing substrate composed of two truncated 
            GFP genes, separated by a drug selection marker, and flanked by 
            mouse hprt targeting sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M
  TITLE     Genetic steps of mammalian homologous repair with distinct mutagenic
            consequences.
  JOURNAL   Mol Cell Biol. 2004 Nov;24(21):9305-16.
  PUBMED    15485900
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-11"; volume: "24(21)"; pages: "9305-16"
COMMENT     Sequence Label: hprtSAGFP
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(85..106)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     434..453
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgtggtatg gctgattatg atctagagtc gcggccgctt tacttgtaca gctcgtccat
       61 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      121 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      181 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      241 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      301 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      361 ctccttgaag tcgatgccct tcagctcgat gcggattacc ctgttatccc tatcgaactt
      421 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      481 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      541 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      601 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      661 ctcgccggac acgctgaact tgtggcctta aagcttgggc tgcaggtcga gggatctcca
      721 taagagaaga gggacagcta tgactgggag tagtcaggag aggag
//
