LOCUS       sequence-56393-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GFP(S65T)-His3MX6
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      45..232
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(100..124)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        307..650
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ggcatggatg aactatacaa atagggcgcg ccacttctaa ataagcgaat ttcttatgat
       61 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
      121 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
      181 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
      241 ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg
      301 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
      361 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
      421 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc
      481 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg
      541 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt
      601 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atggcagaac
      661 cagcccaaaa aaagcaaaaa caaactgttc aggagcgcaa ggcgtttatc tcccgtatca
      721 ctaatgaaac taaaattcaa atcgctattt cgctgaatgg tggttatatt caaataaaag
      781 attcgattct tcctgcaaag aaggatgacg atgtagcttc ccaagctact cagtcacagg
      841 tcatcgatat tcacacaggt gttggctttt tggatcatat gatccatgcg ttggcaaaac
      901 actctggttg gtctcttatt gttgaatgta ttggtgacct gcacattgac gatcaccata
      961 ctaccga
//
