LOCUS       sequence-56400-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GFP(S65T)-His3MX6
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(70..267)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(561..575)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 tggatctgat atcatcgatg aattcgagct cgtttaaact ggatggcggc gttagtatcg
       61 aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata ttactttctg
      121 cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct
      181 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt
      241 gaaaacaaga atctttttat tgtcagtact tcacatcaaa acacctttgg ttgagggaac
      301 gtcattggtg ccattgctag aaatagcttc tcttatggca acagccaaag ccttgaacgc
      361 actctcactt ctgtggtgat cgttgaaacc tctcagacaa tcaacatgca aagtaattct
      421 ggccgcctcc gcgaaacttt ccaaaaagtg tggaatcatt tcagtggata aatcaccaat
      481 catctctctc ttcaatccaa ggtcgattac agcaaatggt ctactagata aatcgactac
      541 ggcacgtgat agcgcctcat ccaatggtgc gaacccagta ccgaatcttt ttacaccacg
      601 gacagcaccc attgcttctt tgaacgcttg ccctaatgcg ataccgcaat cttcggtagt
      661 atggtgatcg tcaatgtgca ggtcaccaat acattcaaca ataagagacc aaccagagtg
      721 ttttgccaac gcatggatca tatgatccaa aaagccaaca cctgtgtgaa tatcgatgac
      781 ctgtgactga gtagcttggg aagctacatc gtcatccttc tttgcaggaa gaatcgaatc
      841 ttttatttga atataaccac cattcagcga aatagcgatt tgaattttag tttcattagt
      901 gatacggga
//
