LOCUS       sequence-56420-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-13Myc-His3MX6
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..540
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      595..782
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(650..674)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaacg gtgaacaaaa gctaatctcc
       61 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggactcgac
      121 ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag
      181 gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg actcgacggt
      241 gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat ctccgaggaa
      301 gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact cgacggtgaa
      361 caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac
      421 ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggactcga cggtgaacaa
      481 aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg
      541 aacggtgaac aaaaattaat caatcactag tgaattcgcg ccacttctaa ataagcgaat
      601 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
      661 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
      721 taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc
      781 ggcagatccg ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc
      841 gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg
      901 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
//
