LOCUS       sequence-56432-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GST-His3MX6
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      44..231
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(99..123)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        306..649
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aatcggatct ggttccgcgt tgaggcgcgc cacttctaaa taagcgaatt tcttatgatt
       61 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
      121 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
      181 gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc
      241 tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg
      301 ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg
      361 catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat
      421 ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct
      481 gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt
      541 agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta
      601 aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca tggcagaacc
      661 agcccaaaaa aagcaaaaac aaactgttca ggagcgcaag gcgtttatct cccgtatcac
      721 taatgaaact aaaattcaaa tcgctatttc gctgaatggt ggttatattc aaataaaaga
      781 ttcgattctt cctgcaaaga aggatgacga tgtagcttcc caagctactc agtcacaggt
      841 catcgatatt cacacaggtg ttggcttttt ggatcatatg atccatgcgt tggcaaaaca
      901 ctctggttgg tctct
//
