LOCUS       sequence-56494-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-kanMX6-PGAL1-GST
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..702)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(69..91)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(743..766)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gctgcaggtc gacggatctg gcgcgcctca acgcggaacc agatccgatt ttggaggatg
       61 gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga
      121 tttcaagtac ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt
      181 tgggaacgca tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa
      241 catgaagtca ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc
      301 ttcgaacatt ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa
      361 gtctttacta tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag
      421 cattgaaatc tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat
      481 ataacgtatg atggccatag actgtgttaa tttaacatca ccatcaatat aataaggaag
      541 attgggaaac tccaaaccca attcaaactt tttgtttcgc catttatcac cttcatcgcg
      601 ctcatacaaa tgctcttcat atttttcttc aagatattcc aaaagaagtc gagtgggttg
      661 cacaaggccc ttaattttcc aataacctag tataggggac atattaatta aagacatttt
      721 gagatccggg ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt
      781 gatactttta ttacatttga ataagaagta atacaaaccg aaaatgttga aagtattagt
      841 taaagtggtt atgcagtttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt
      901 cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcatta
//
