LOCUS       sequence-56530-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-GFP
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(50..237)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     158..182
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(301..321)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     916..944
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgctgcaggt cgacggatct atattaccct gttatcccta gcggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catactatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcccta tttgtatagt tcatccatgc catgtgtaat cccagcagct
      301 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      361 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      421 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      481 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta
      541 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt
      601 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag
      661 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc
      721 ttgaaaaagt catgccgttt catatgatct gggtatcttg aaaagcattg aacaccataa
      781 gtgaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      841 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg
      901 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagt
//
