LOCUS       sequence-56531-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-GFP
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             44..52
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(132..806)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(807..954)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 cactagtgga tctgatatca tcgatgaatt cgagctcgtt taaacctcct tacgcatctg
       61 tgcggtattt cacaccgcat agatcggcaa gtgcacaaac aatacttaaa taaatactac
      121 tcagtaataa cctatttctt agcatttttg acgaaatttg ctattttgtt agagtctttt
      181 acaccatttg tctccacacc tccgcttaca tcaacaccaa taacgccatt taatctaagc
      241 gcatcaccaa cattttctgg cgtcagtcca ccagctaaca taaaatgtaa gctttcgggg
      301 ctctcttgcc ttccaaccca gtcagaaatc gagttccaat ccaaaagttc acctgtccca
      361 cctgcttctg aatcaaacaa gggaataaac gaatgaggtt tctgtgaagc tgcactgagt
      421 agtatgttgc agtcttttgg aaatacgagt cttttaataa ctggcaaacc gaggaactct
      481 tggtattctt gccacgactc atctccatgc agttggacga tatcaatgcc gtaatcattg
      541 accagagcca aaacatcctc cttaggttga ttacgaaaca cgccaaccaa gtatttcgga
      601 gtgcctgaac tatttttata tgcttttaca agacttgaaa ttttccttgc aataaccggg
      661 tcaattgttc tctttctatt gggcacacat ataataccca gcaagtcagc atcggaatct
      721 agagcacatt ctgcggcctc tgtgctctgc aagccgcaaa ctttcaccaa tggaccagaa
      781 ctacctgtga aattaataac agacatactc caagctgcct ttgtgtgctt aatcacgtat
      841 actcacgtgc tcaatagtca ccaatgccct ccctcttggc cctctccttt tcttttttcg
      901 accgaattaa ttcttaatcg gcaaaaaaag aaaagctccg gatcaagatt gtacagatct
      961 gtaaagagcc
//
