LOCUS       sequence-56532-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-GFP
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..491)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(56..79)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    374..491
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        586..733
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 ttcttctcct ttactgttaa ttaaagacat tttgagatcc gggttttttc tccttgacgt
       61 taaagtatag aggtatatta acaatttttt gttgatactt ttattacatt tgaataagaa
      121 gtaatacaaa ccgaaaatgt tgaaagtatt agttaaagtg gttatgcagt ttttgcattt
      181 atatatctgt taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg
      241 ctaaaaaact aatcgcatta tcatcctatg gttgttaatt tgattcgttc atttgaaggt
      301 ttgtggggcc aggttactgc caatttttcc tcttcataac cataaaagct agtattgtag
      361 aatctttatt gttcggagca gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg
      421 gtgaagacga ggacgcacgg aggagagtct tccttcggag ggctgtcacc cgctcggcgg
      481 cttctaatcc gtacttcaat atagcaatga gcagttaagc gtattactga aagttccaaa
      541 gagaaggttt ttttaggcta agataatggg gctctttaca gatctgtaca atcttgatcc
      601 ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa aaggagaggg
      661 ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa
      721 ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt
      781 ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg ctgacttgct
      841 gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg caaggaaaat
      901 ttcaagtctt gtaaaagcat ataaaaatag ttcagg
//
