LOCUS       sequence-56571-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Wayne N, Bolon DN
  TITLE     Dimerization of Hsp90 is required for in vivo function. Design and 
            analysis of monomers and dimers.
  JOURNAL   J Biol Chem. 2007 Nov 30;282(48):35386-95. Epub 2007 Oct 1.
  PUBMED    17908693
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-11-30"; volume: "282(48)"; pages: "35386-95. 
            Epub 2007 Oct 1"
COMMENT     Sequence Label: pRNDM
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(49..392)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(445..463)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(446..463)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(685..704)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     804..826
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(864..882)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttggaattt aatcgcggcc tcgaaacgtg agtcttttcc ttacccatgg ttgtttatgt
       61 tcggatgtga tgtgagaact gtatcctagc aagattttaa aaggaagtat atgaaagaag
      121 aacctcagtg gcaaatccta accttttata tttctctaca ggggcgcggc gtggggacaa
      181 ttcaacgcgt ctgtgagggg agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa
      241 tttttgcttc gcgccgtgcg gccatcaaaa tgtatggatg caaatgatta tacatgggga
      301 tgtatgggct aaatgtacgg gcgacagtca catcatgccc ctgagctgcg cacgtcaaga
      361 ctgtcaagga gggtattctg ggcctccatg tcgctggccg ggtgacccgg cggggacgag
      421 gcaagctaaa cagatcggcc gcgttctata gtgtcaccta aatcgtatgt gtatgataca
      481 taaggttatg tattaattgt agccgcgttc taacgacaat atgtccatat atgcgtatat
      541 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat
      601 caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga
      661 aaaggtggta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      721 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      781 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      841 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      901 gataataatg gtttcttaga cggatcgctt gcctgta
//
