LOCUS       sequence-56675-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pUG6SP-tTA'
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             93..713
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(166..185)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
       61 ggaccgatcc agcctccgcg gccccaattc atatgtctag attagataaa agtaaagtga
      121 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
      181 tcgcccagaa gcttggtgta gagcagccta cactgtattg gcatgtaaaa aataagcggg
      241 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
      301 taaaagggga aagctggcaa gattttttac gcaataacgc taaaagtttt agatgtgctt
      361 tactaagtca tcgcaatgga gcaaaagtac attcagatac acggcctaca gaaaaacagt
      421 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaacg
      481 cgttatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
      541 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
      601 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
      661 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca
      721 gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga gggcctgctc gatctcccgg
      781 acgacgacgc
//
