LOCUS       sequence-56744-e        8884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-(1-924)
FEATURES             Location/Qualifiers
     source          1..8884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4478..4495)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4484..4708
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4754..5182
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4841..4860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5051..5072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5191..5211)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5196..5525
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5376..5511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5438..5457
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5592..6386
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5646..5665)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6256..6275
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6560..6681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6597..6616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6651..6670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6730..6746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6730..6746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6743..6765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6754..6770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6778..6784,6785..6802,6803..6808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6823..6844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6961..6978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7132..7717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7212..7231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7888..8679,8680..8748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8511..8530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8749..8853)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaaca gaactgactt agatccacgt
     3061 gtcctgtcca actctggttg gggacagact cctattaagc agaatactgc ctgggataca
     3121 gaaacatcac ctagagggga acgaaagact gacaatggga cagaggcctg gggaagctct
     3181 gcaacacaga cttttaactc aggggcatgt atagataaga ctagccctaa tggtaatgat
     3241 acctcatctg tatcagggtg gggcgatccc aaacctgctc tgaggtgggg agattccaaa
     3301 ggctcaaact gccagggggg gtgggaagat gattctgctg ctacaggaat ggtcaagagc
     3361 aatcagtggg ggaattgcaa agaggagaag gctgcatgga atgactcgca aaagaataaa
     3421 cagggatggg gtgatggaca aaaatcaagc caagggtggt ctgtttctgc cagtgataac
     3481 tggggagaaa cttcaaggaa taaccattgg ggtgaggcca ataagaaatc cagctcagga
     3541 ggtagtgaca gtgacaggtc cgtttccggt tggaacgaac ttggtaaaac tagttctttc
     3601 acttggggaa acaacataaa tccaaataat tcatcaggat gggatgaatc ttctaaacct
     3661 actccttccc agggatgggg agaccctcca aagtctaatc agtctctagg ttggggagat
     3721 tcgtcaaagc cagtcagctc tccagactgg aacaagcaac aagacattgt tggatcttgg
     3781 ggaatcccac cagctacagg caaacctcct ggtacaggct ggctgggggg acctatacca
     3841 gccccagcaa aagaagaaga acccacaggc tgggaggaac catccccaga atctatacgt
     3901 cgcaaaatgg agattgatga tggaacttca gcttggggag atccaagcaa atacaactac
     3961 aaaaatgtga acatgtggaa caaaaacgtc ccaaatggca acagccgttc agaccagcaa
     4021 gcacaggtac atcagctgct aacgcctgca agtgccatct caaacaaaga ggcaagcagt
     4081 ggctctggct ggggtgagcc ctggggggag ccttctactc cagccacaac tgtggataat
     4141 ggtacttcag catggggtaa gcccatagac agtggtccca gctgggggga acccattgct
     4201 gcggcatcca gcacatccac gtggggctcc agctctgttg gtccacaagc attaagcaaa
     4261 tctgggccaa aatctatgca agatggctgg tgtggtgatg atatgccatt gcctggaaat
     4321 cgccccactg gctgggaaga ggaagaggat gtggagattg gaatgtggaa tagtaattca
     4381 tctcaagagc ttaactcatc tttaaattgg ccaccatata caaagaaaat gtcatcgaag
     4441 ggttgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     4501 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4561 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4621 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4681 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     4741 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4801 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4861 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4921 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4981 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5041 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5101 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5161 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     5221 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5281 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5341 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5401 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5461 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5521 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     5581 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5641 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5701 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5761 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5821 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5881 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5941 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     6001 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     6061 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     6121 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6181 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6241 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6301 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6361 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6421 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6481 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6541 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6601 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6661 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6721 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6781 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6841 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6901 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6961 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7021 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7081 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7141 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7201 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7261 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7321 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7381 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7441 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7501 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7561 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7621 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     7681 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7741 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7801 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7861 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7921 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7981 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8041 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8101 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8161 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8221 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8281 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8341 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8401 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8461 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8521 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8581 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8641 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8701 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8761 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8821 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8881 cgtc
//
