LOCUS       sequence-56890-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pHYDuet183
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaataa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcct aggacagtac aaacttatcc tgaacggtaa
      121 aaccctgaaa ggtgaaacca ccaccgaagc tgttgacgct gctaccgcgg aaaaagtttt
      181 caaacagtac gctaacgaca acggtgttga cggtgaatgg acctacgacg acgctaccaa
      241 aacctacacc gtaacggaag gatccgggaa gtgctttgct agggataccg aagtttatta
      301 tgaaaacgat actgtaccac acatggaatc aattgaggag atgtatagta aatacgcctc
      361 gatgaatggg gaattaccat tcgataatgg ttacgcagtt ccattagata atgtctttgt
      421 gtacacgttg gacatcgcta gtggtgaaat taagaaaacg agggcttcat acatctatcg
      481 cgagaaggtt gagaagctca ttgagataaa actatccagt ggatactccc ttaaggttac
      541 tccctctcac ccagttctcc tctttagaga tggcctgcaa tgggtccccg ctgctgaagt
      601 taaacctgga gatgttgttg ttggtgttag ggaagaggta ctgagaagaa gaataatatc
      661 caaaggagaa cttgaattcc atgaggtttc ctcggtaagg ataatagatt acaataactg
      721 ggtctacgac cttgtaattc cggaaaccca caacttcata gctcccaatg gacttgttct
      781 ccataatact cagctaggta cccagtacaa acttatcctg aacggtaaaa ccctgaaagg
      841 tgaaaccacc accgaagctg ttgacgctgc taccgcggaa a
//
