LOCUS       sequence-56892-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet26
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaataa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcct aggacagtac aaacttatcc tgaacggtaa
      121 aaccctgaaa ggtgaaacca ccaccgaagc tgttgacgct gctaccgcgg aaaaagtttt
      181 caaacagtac gctaacgaca acggtgttga cggtgaatgg acctacgacg acgctaccaa
      241 aacctacacc gtaacggaag gatccaagtg cgtatcaggt gaaacaccag tttaccttgc
      301 ggatggcaag acaataaaaa taaaggatct atacagttct gagagaaaaa aagaagataa
      361 cattgttgag gctggttcgg gagaagagat aatacatcta aaagatccca ttcaaatata
      421 ttcttatgtg gacgggacca tagtcaggag cagatcaaga cttctataca agggcaagag
      481 ctcttatctt gtgaggatag aaactattgg cggaagatcg gtaagcgtta caccagttca
      541 caaactcttt gtccttacgg aaaagggtat cgaagaggtt atggcctcca acctaaaggt
      601 aggcgacatg attgctgctg tagcagaaag cgaatctgaa gcaagagact gcggaatgag
      661 cgaggaatgc gtgatggaag cagaagttta tacgtcactt gaagcgacat tcgatagagt
      721 aaagtctata gcgtacgaga agggtgattt tgatgtatac gatctttccg taccagaata
      781 cggcaggaac tttataggcg gagaaggact tctcgtactt cacaacacgg ttataggtac
      841 ccagtacaaa cttatcctga acggtaaaac cctgaaaggt gaaaccacca ccgaagctgt
      901 tgacgctgct accgcggaaa aa
//
