LOCUS       sequence-56910-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase from mouse PGK promoter in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     Rajewsky pPGK-Cre-bpA
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPGK-Cre-bpA
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5..21
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        46..545
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(81..104)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     478..497
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             570..590
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccctcgaggt cgacggtatc gataagcttg atatcgaatt ctaccgggta ggggaggcgc
       61 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
      121 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
      181 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
      241 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
      301 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
      361 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
      421 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
      481 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
      541 tttcgacctg cagctcgagg tcgaccatgc ccaagaagaa gaggaaggtg tccaatttac
      601 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      661 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      721 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      781 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      841 taaaaactat ccagcaacat ttgggccagc taaacatg
//
