LOCUS       sequence-56933-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Voytas Golden Gate TALEN assembly 
            incorporating a CAG promoter for mammalian expression and a T7 
            promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain
            (ELD)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Hermann M, Cermak T, Voytas DF, Pelczar P
  TITLE     Mouse genome engineering using designer nucleases.
  JOURNAL   J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930.
  PUBMED    24747757
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-04-2"; volume: "(86). doi"; pages: " 
            10.3791/50930"
COMMENT     Sequence Label: pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    765..786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(801..806,807..824,825..831)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    839..855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     844..866
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     863..879
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     863..879
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctcgccgttg ttgaacttgc gccgcacctc ctccagtgtc agggtgccgg ctttgatcat
       61 ctcgccgccg atcagcagct cctccacgct cagcacggcg ccattgcagt tggtgatgtg
      121 gttcagcctg gtcagctggg ccttgtagtt gcccttgaag tggccgctca cgaacaggaa
      181 cttgaactcg gtcacgctgc tagggtacac cttccaccac tcgttggggt tgaggtgctt
      241 atcccgtgtc tggttctcct ccacgtatct ctccatctcg tcggcctggc cgataggcag
      301 attgtagccg ccgctgtagg cctttgtgtc cacgatcacg ccgtaatcga tggggctgcc
      361 cactgtatag atggcgccgt caggctttct gcttccgccc aggtgctctc ccctgtagcc
      421 gtacaccttc atgaagaact ccatcacctt catctccagg atgcggtcct gggtggggtt
      481 cctggcgatc tcgatcagct cgatgtactc gtggggcacg tacttcagct tgtgccgcag
      541 ctcggacttc ttctcctcca gctcgctctt caccagctgg gatcccgcaa cgcgatggga
      601 cgtgcgttcg ccaatacggc gattgactct tctgatcaat tccggcgcgt gcggcaatcc
      661 ctttttcact gcatccatgg caggacgtcc gccgaggcag gccaaggcga cgaggtggtc
      721 gttggagacg accggtggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      781 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      841 gtgagcggat aacaatttca cacaggaaac agctatgacc atga
//
