LOCUS       sequence-56964-e        1396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   O'Neill PR, Karunarathne WK, Kalyanaraman V, Silvius JR, Gautam N
  TITLE     G-protein signaling leverages subunit-dependent membrane affinity to
            differentially control betagamma translocation to intracellular 
            membranes.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3568-77. doi: 
            10.1073/pnas.1205345109. Epub 2012 Dec 3.
  PUBMED    23213235
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1205345109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-18- 18"; volume: "109"; issue: "51"; pages: 
            "E3568-77"
COMMENT     Sequence Label: Venus-G protein Beta2 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..302
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     239..260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gnggggngtg gggttaactt aagcttggta ccgagctcgg atccaccatg gacaagcaga
       61 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
      121 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      181 accactacct gagctaccag tccaaactga gcaaagaccc caacgagaag cgcgatcaca
      241 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
      301 agggcggatc cgggggaggg agtgagctgg agcaactgag acaggaggcc gagcagctcc
      361 ggaaccagat ccgggatgcc cgaaaagcat gtggggactc aacactgacc cagatcacag
      421 ctgggctgga cccagtgggg agaatccaga tgaggacccg gaggaccctc cgtgggcacc
      481 tggcaaagat ctatgccatg cactggggga ccgactcaag gctgctggtc agcgcctccc
      541 aggatgggaa gctcatcatc tgggacagct acaccaccaa caaggtccac gccatcccgc
      601 tgcgctcctc ctgggtaatg acctgtgcct acgcgccctc agggaacttt gtggcctgtg
      661 gggggttgga caacatctgc tccatctaca gcctcaagac ccgcgagggc aacgtcaggg
      721 tcagccggga gctgcctggc cacactgggt acctgtcgtg ttgccgcttc ctggatgaca
      781 accaaatcat caccagctct ggggatacca cctgtgccct gtgggacatt gagacaggcc
      841 agcagacagt gggttttgct ggacacagtg gggatgtgat gtccctgtcc ctggcccccg
      901 atggccgcac gtttgtgtca ggcgcctgtg atgcctctat caagctgtgg gacgtgcggg
      961 attccatgtg ccgacagacc ttcatcggcc atgaatccga catcaatgca gtggctttct
     1021 tccccaacgg ctacgccttc accaccggct ctgacgacgc cacgtgccgc ctcttcgacc
     1081 tgcgggccga tcaggagctc ctcatgtact cccatgacaa catcatctgt ggcatcacct
     1141 ctgttgcctt ctcgcgcagc ggacggctgc tgctcgctgg ctacgacgac ttcaactgca
     1201 acatctggga tgccatgaag ggcgaccgtg caggagtcct cgctggccac gacaaccgcg
     1261 tgagctgcct cggggtcacc gacgatggca tggctgtggc cacgggctcc tgggactcat
     1321 tcctcaagat ctggaactaa ctcgagtcta gagggccggg ttaaacccgc tgatcagcct
     1381 tganttnccc canntt
//
