LOCUS       sequence-56969-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength intracellular glutamate sensor constructed from 
            E. coli Gltl and cpGFP. For bacterial cytoplasmic expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, 
            Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter 
            ER, Demb JB, Gan WB, Hires SA, Looger LL
  TITLE     An optimized fluorescent probe for visualizing glutamate 
            neurotransmission.
  JOURNAL   Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 
            2013 Jan 13.
  PUBMED    23314171
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2333"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "162-70"
COMMENT     Sequence Label: pRSET.GltI253-cpGFP.L1LV/L2NP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..110
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(114..122,123..137)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcgg ggttctcatc
       61 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
      121 acgacgatga cgataaggat cgatggggat ccgccgcagg cagcacgctg gacaaaatcg
      181 ccaaaaacgg tgtgattgtc gtcggtcacc gtgaatcttc agtgcctttc tcttattacg
      241 acaatcagca aaaagtggtg ggttactcgc aggattactc caacgccatt gttgaagcag
      301 tgaaaaagaa actcaacaaa ccggacttgc aggtaaaact gattccgatt acctcacaaa
      361 accgtattcc actgctgcaa aacggcactt tcgattttga atgtggttct accaccaaca
      421 acgtcgaacg ccaaaaacag gcggctttct ctgacactat tttcgtggtc ggtacgcgcc
      481 tgttgaccaa aaagggtggc gatatcaaag attttgccaa cctgaaagac aaagccgtag
      541 tcgtcacttc cggcactacc tctgaagttt tgctcaacaa actgaatgaa gagcaaaaaa
      601 tgaatatgcg catcatcagc gccaaagatc acggtgactc tttccgcacc ctggaaagcg
      661 gtcgtgccgt tgcctttatg atggatgacg tgctgctggc cggtgaacgt gcgaaagcga
      721 agaaaccaga caactgggaa atcgtcggca agccgcagtc tcaggaggcc tacggttgta
      781 tgttgcgtaa agatgatccg cagttcaaaa agctgatgga tgacaccatc gctcaggtgc
      841 agacctccgg tgaagcggaa aaatggtttg ataagtggtt caaaaatcca attctggtga
      901 gccacaacgt ctatatcatg gccgacaagc agaggaacgg catcaaggcg a
//
