LOCUS       sequence-57030-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber
            ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
  TITLE     Exploiting a natural conformational switch to engineer an 
            interleukin-2 'superkine'.
  JOURNAL   Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.
  PUBMED    22446627
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-25- 25"; volume: "484"; issue: "7395"; 
            pages: "529-33"
COMMENT     Sequence Label: D10 pAcGP67a
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     125..184
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             599..622
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             637..681
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             682..699
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
       61 gcgcggatct atgctactag taaatcagtc acaccaaggc ttcaataagg aacacacaag
      121 caagatggta agcgctattg ttttatatgt gcttttggcg gcggcggcgc attctgcctt
      181 tgcggcggat cccgcaccta cttcaagttc tacaaagaaa acacagctac aactggagca
      241 tttacttctg gatttacaga tgattttgaa tggaattaat aattacaaga atcccaaact
      301 caccaggatg ctcacattta agttttacat gcccaagaag gccacagaac tgaaacatct
      361 tcagtgtcta gaagaagaac tcaaacctct ggaggaagtg ctaaatttag ctcacagcaa
      421 aaactttcac ttcgatccca gggacgtcgt cagcaatatc aacgtattcg tcctggaact
      481 aaagggatct gaaacaacat tcatgtgtga atatgctgat gagacagcaa ccattgtaga
      541 atttctgaac agatggatta ccttttgtca aagcatcatc tcaacactaa ctggcggcca
      601 tcatcaccac catcaccacc attaatgagc ggccgcggtc ttaatgatat tttcgaggct
      661 cagaagattg agtggcatga acatcatcat catcatcatt aaagatctga tcctttcctg
      721 ggacccggca agaaccaaaa actcactctc ttcaaggaaa tccgtaatgt taaacccgac
      781 acgatgaaag cttgtcgttg gatggaaagg aaaagagttc tacaggg
//
