LOCUS       sequence-57091-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon optimized Cas9 nuclease for genome 
            engineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE,
            Church GM
  TITLE     RNA-Guided Human Genome Engineering via Cas9.
  JOURNAL   Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033
  PUBMED    23287722
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-02-15- 15"; volume: "339"; 
            issue: "6121"; pages: "823-6"
COMMENT     Sequence Label: hCas9
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..51)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttactaaccg gtagggatcg aaccctttca caccttcctc ttcttcttgg ggtcagccct
       61 gctgtctcca ccgagctgag agaggtcgat tcttgtttca tagagccccg taattgactg
      121 atgaatcagt gtggcgtcca ggacctcctt tgtagaggtg taccgctttc tgtctatggt
      181 ggtgtcgaag tacttgaagg ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat
      241 aatgttttct gcctgctccc tgatgggctt atccctgtgc ttattgtaag cagaaagcac
      301 cttatcgagg ttagcgtcgg cgaggatcac tcttttggag aattcgctta tttgctcgat
      361 gatctcatca aggtagtgtt tgtgttgttc cacgaacagc tgcttctgct cattatcttc
      421 gggagaccct ttgagctttt catagtggct ggccagatac aagaaattaa cgtatttaga
      481 gggcagtgcc agctcgttac ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg
      541 gccgttttca agctcaaaga gagagtactt gggaagctta atgatgaggt cttttttgac
      601 ctctttatat cctttcgcct cgagaaagtc gatggggttt ttttcgaagc ttgatcgctc
      661 catgattgtg atgcccagca gttccttgac gcttttgagt tttttagact tccctttctc
      721 cactttggcc acaaccagta cactgtaagc gactgtagga gaatcgaatc cgccgtattt
      781 cttggggtcc caatcttttt tgcgtgcgat cagcttgtcg ctgttccttt tcgggaggat
      841 actttccttg gagaagcctc cggtctgtac ttcggtcttt ttaacgatgt tcacctgcgg
      901 catggacagg accttccgga ctgtcgcgaa atccctaccc ttgtcccaca cgatttctcc
      961 tgtttctccg tttgtt
//
