LOCUS       sequence-57204-e        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITPK1A
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcagacctt tctgaaaggg aagagagttg gctactggct gagcgagaag
      121 aaaatcaaga agctgaattt ccaggccttc gccgagctgt gcaggaagcg agggatggag
      181 gttgtgcagc tgaaccttag ccggccgatc gaggagcagg gccccctgga cgtcatcatc
      241 cacaagctga ctgacgtcat ccttgaagcc gaccagaatg atagccagtc cctggagctg
      301 gtgcacaggt tccaggagta catcgatgcc caccctgaga ccatcgtcct ggacccgctc
      361 cctgccatca gaaccctgct tgaccgctcc aagtcctatg agctcatccg gaagattgag
      421 gcctacatgg aagacgacag gatctgctcg ccacccttca tggagctcac gagcctgtgc
      481 ggggatgaca ccatgcggct gctggagaag aacggcttga ctttcccatt catttgcaaa
      541 accagagtgg ctcatggcac caactctcac gagatggcta tcgtgttcaa ccaggagggc
      601 ctgaacgcca tccagccacc ctgcgtggtc cagaatttca tcaaccacaa cgccgtcctg
      661 tacaaggtgt tcgtggttgg cgagtcctac accgtggtcc agaggccctc actcaagaac
      721 ttctccgcag gcacatcaga ccgtgagtcc atcttcttca acagccacaa cgtgtcaaag
      781 ccggagtcgt catcggtcct gacggagctg gacaagatcg agggcgtgtt cgagcggccg
      841 agcgacgagg tcatccggga gctctcccgg gccctgcggc aggcactggg cgtgtcactc
      901 ttcggcatcg acatcatcat caacaaccag acagggcagc acgccgtcat tgacatcaat
      961 gccttcccag gctacgaggg cgtgagcgag ttcttcacag acctcctgaa ccacatcgcc
     1021 actgtcctgc agggccagag cacagccatg taacagtaaa ggtggatacg gatccgaa
//
