LOCUS       sequence-57223-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPG7A
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgggagtcag cttcaaagac gtggcaggaa tgcacgaagc caaactggaa
      121 gtccgcgagt ttgtggatta tctgaagagc ccagaacgct tcctccagct tggcgccaag
      181 gtcccaaagg gcgcactgct gctcggcccc cccggctgtg ggaagacgct gctggccaag
      241 gcggtggcca cggaggctca ggtgcccttc ctggcgatgg ccggcgcaga gttcgtggag
      301 gtcattggag gcctcggcgc tgcccgtgtg cggagcctct ttaaggaagc ccgagcccgg
      361 gccccctgca tcgtctacat cgatgagatc gacgcggtgg gcaagaagcg ctccaccacc
      421 atgtccggct tctccaacac ggaggaggag cagacgctca accagcttct ggtagaaatg
      481 gatggaatgg gtaccacaga ccatgtcatc gtcctggcgt ccacgaaccg agctgacatt
      541 ttggacggtg ctctgatgag gccaggccga ctggaccggc acgtcttcat tgatctcccc
      601 acgctgcagg agaggcggga gatttttgag cagcacctga agagcctgaa gctgacccag
      661 tccagcacct tttactccca gcgtctggca gagctgacac caggattcag tggggctgac
      721 atcgccaaca tctgcaatga ggctgcgctg cacgcggcgc gggagggaca cacttccgtg
      781 cacactctca acttcgagta cgccgtggag cgcgtcctcg cagggactgc caaaaagagt
      841 aagatcctgt ccaagtgaca gtaaaggtgg atacggatcc gaa
//
